Reporter

Part:BBa_K2541401:Design

Designed by: Shuting Zheng   Group: iGEM18_Jilin_China   (2018-10-05)


sfGFP(BbsI free)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 13


Design Notes

sfGFP(BbsI free) has two mutations from superfolder GFP(BBa_I746916)—from 5’-gaagac-3’ to 5’-gaggat-3’, which does not change the amino acid encoded by this sequence.

Source

sfGFP(BbsI free) has two mutations from superfolder GFP(BBa_I746916).

References

[1]Southward CM, Surette MG. 2002. The dynamic microbe: green fluorescent protein brings bacteria to light. Mol. Microbiol. 45:1191–1196.

[2]Cormack BP, Valdivia RH, Falkow S. 1996. FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173:33–38.

[3]Pédelacq J-D, Cabantous S, Tran T, Terwilliger TC, Waldo GS. 2006. Engineering and characterization of a superfolder green fluorescent protein. Nat. Biotechnol. 24:79 –88.